Regina C. LaRocque, Jason B. Harris, Michelle Dziejman, Xiaoman Li et al.
ABSTRACT Understanding gene expression by bacteria during the actual course of human infection may provide important insights into microbial pathogenesis. In this study, we evaluated the transcriptional profile of Vibrio cholerae , the causative agent of cholera, in clinical specimens from cholera p...
Uzma Z. Rizvi
Abstract Acknowledgement I would like to thank my colleagues and friends Praveena Gullapalli and Benjamin Porter for the comments and insights that have helped shaped this piece in its initial stages. Additionally, this work has benefited from my conversations with Sandra Scham. I would also like to...
R. Gray Huffman, Andrew Leduc, Christoph Wichmann, Marco Di Gioia et al.
Major aims of single-cell proteomics include increasing the consistency, sensitivity and depth of protein quantification, especially for proteins and modifications of biological interest. Here, to simultaneously advance all these aims, we developed prioritized Single-Cell ProtEomics (pSCoPE). pSCoPE...
Debasish Saha, Regina C. LaRocque, Ashraful Islam Khan, Jason B. Harris et al.
The serum vibriocidal antibody is the only recognized predictor of protection from cholera, but no seroepidemiological data have been gathered since the emergence of Vibrio cholerae O139. We assessed the association between the vibriocidal antibody titer and protection from cholera in an endemic set...
Daniel T. Leung, Taufiqur Rahman Bhuiyan, Naoshin Sharmin Nishat, Mohammad Rubel Hoq et al.
BACKGROUND: Mucosal Associated Invariant T (MAIT) cells are innate-like T cells found in abundance in the intestinal mucosa, and are thought to play a role in bridging the innate-adaptive interface. METHODS: We measured MAIT cell frequencies and antibody responses in blood from patients presenting w...
Ashfaqul Alam, Regina C. LaRocque, Jason B. Harris, Cecily Vanderspurt et al.
It has previously been shown that passage of Vibrio cholerae through the human intestine imparts a transient hyperinfectious phenotype that may contribute to the epidemic spread of cholera. The mechanism underlying this human-passaged hyperinfectivity is incompletely understood, in part due to inher...
Firas S. Midani, Ana A. Weil, Fahima Chowdhury, Yasmin Ara Begum et al.
Background: Cholera is a public health problem worldwide, and the risk factors for infection are only partially understood. Methods: We prospectively studied household contacts of patients with cholera to compare those who were infected to those who were not. We constructed predictive machine learni...
Elinor K. Karlsson, Jason B. Harris, Shervin Tabrizi, Atiqur Rahman et al.
As an ancient disease with high fatality, cholera has likely exerted strong selective pressure on affected human populations. We performed a genome-wide study of natural selection in a population from the Ganges River Delta, the historic geographic epicenter of cholera. We identified 305 candidate s...
Russell A. Johnson, Taher Uddin, Amena Aktar, M. Mohasin et al.
Immunity against Vibrio cholerae O1 is serogroup specific, and serogrouping is defined by the O-specific polysaccharide (OSP) part of lipopolysaccharide (LPS). Despite this, human immune responses to V. cholerae OSP have not previously been characterized. We assessed immune responses against V. chol...
Sweta Patel, Mohammad Arif Rahman, M. Mohasin, M. Asrafuzzaman Riyadh et al.
Vibrio cholerae O1 causes cholera, a dehydrating diarrheal disease. We have previously shown that V. cholerae-specific memory B cell responses develop after cholera infection, and we hypothesize that these mediate long-term protective immunity against cholera. We prospectively followed household con...
Mohammad Murshid Alam, M. Asrafuzzaman Riyadh, Kaniz Fatema, Mohammad Arif Rahman et al.
The mediators of protective immunity against cholera are currently unknown, but memory B-cell responses may play a central role in facilitating long-term and anamnestic responses against Vibrio cholerae, the cause of cholera. We compared memory B-cell responses in adults with natural cholera in Bang...
Jason B. Harris, Michael J. Podolsky, Taufiqur Rahman Bhuiyan, Fahima Chowdhury et al.
BACKGROUND: Infection with intestinal helminths is common and may contribute to the decreased efficacy of Vibrio cholerae vaccines in endemic compared to non-endemic areas. However, the immunomodulatory effects of concomitant intestinal parasitic infection in cholera patients have not been systemati...
Tahmina Shirin, Taufiqur Rahman Bhuiyan, Richelle C. Charles, Shaheena Amin et al.
OBJECTIVES: Studies on serological responses following coronavirus disease-2019 (COVID-19) have been published primarily in individuals who are moderately or severely symptomatic, but there are few data from individuals who are mildly symptomatic or asymptomatic. METHODS: We measured IgG, IgM, and I...
Robert C. Kauffman, Taufiqur Rahman Bhuiyan, Rie Nakajima, Leslie M. Mayo-Smith et al.
We characterized the acute B cell response in adults with cholera by analyzing the repertoire, specificity, and functional characteristics of 138 monoclonal antibodies (MAbs) generated from single-cell-sorted plasmablasts. We found that the cholera-induced responses were characterized by high levels...
Eric J. Nelson, Ashrafuzzaman Chowdhury, Jason B. Harris, Yasmin Ara Begum et al.
At the International Centre for Diarrhoeal Disease Research, Bangladesh, one-half of the rice-water stool samples that were culture-positive for Vibrio cholerae did not contain motile V. cholerae by standard darkfield microscopy and were defined as darkfield-negative (DF(-)). We evaluated the host a...