Dr. Md. Shahidul Islam, Alf A. Lindberg
A combination of immunomagnetic separation (IMS) and a polymerase chain reaction (PCR) procedure was used for direct isolation and identification of Shigella dysenteriae type 1 and Shigella flexneri from feces. Immunomagnetic particles were coated with monoclonal antibody MASFB, which is specific fo...
Dilara Islam, Alf A. Lindberg, Birger Christensson
We have compared the influence of sample preparation upon the level of surface expression of T, B, and NK cell-related antigens as assessed by flow cytometry. Lysed whole blood (WBL), Ficoll-Paque separated peripheral blood lymphocyte (F-PBL), and frozen peripheral blood lymphocyte (Fr-PBL) were ana...
Nils Carlin, Mustafizur Rahman, David A. Sack, Ayesha Zaman et al.
A panel of 10 mouse and rat monoclonal antibodies specific for different type- and group-specific O-antigenic determinants of Shigella flexneri lipopolysaccharide was used to serotype 240 isolates of S. flexneri from Bangladesh. Three immunoglobulin M antibodies were used in a direct slide agglutina...
Dilara Islam, Pradip Kumar Bardhan, Alf A. Lindberg, Birger Christensson
Immunophenotypic changes in peripheral blood lymphocytes (T, B, and NK cells) in patients during shigellosis was characterized by using triple-color flow cytometry. Eleven Shigella dysenteriae 1-infected adult patients (SDIP), 11 Shigella flexneri-infected adult patients (SFIP), 15 age- and sex-matc...
Rubhana Raqib, Å. Ljungdahl, Alf A. Lindberg, Jan Andersson
In healthy controls (n = 8) living in shigella endemic areas, accumulation of interferon gamma (IFN gamma) in the epithelial lining was seen in the rectal tissues. At the single cell level, however, few or no IFN gamma protein producing cells or mRNA expressing cells were detected at that site indic...
Rubhana Raqib, Å. Ljungdahl, Alf A. Lindberg, Bengt Wretlind et al.
In our study, infection with Shigella dysenteriae type 1 (n = 16) or Shigella flexneri in adults (n = 5) was associated with a gradual accumulation of mRNA for interleukin (IL)-1 beta, tumor necrosis factor (TNF)-alpha, IL-6, transforming growth factor-beta, IL-10, IL-4, TNF-beta, interferon (IFN)-g...
An Li, Phung Dac Cam, Dilara Islam, Nguyễn Bình Minh et al.
The live, auxotrophic Shigella flexneri vaccine strain SFL124 was given in a single dose of 10(7), 10(8) or 10(9) colony forming units (cfu), respectively, to each of three groups of 10 Vietnamese children aged 9-14 years. The vaccine was well tolerated by all the children without any severe side ef...
Dilara Islam, Bengt Wretlind, Alf A. Lindberg, Birger Christensson
A sequential activation of T cells in peripheral blood during shigello sis has been observer (D. Islam, P.K. Bradham, A. A. Lindberg, and B. Christensson, Infect. Immun 63:2941-2949, 1995). To further investigate the cellular response during the course of Shigella infection, changes in the T-cell re...
Rubhana Raqib, Finn P. Reinholt, PRADEEP KUMAR Bardhan, Anders Kärnell et al.
Expression of HLA-DR antigens and infiltration of T-lymphocyte subsets (CD4, CD8), cell activation marker (CD25), B cells (CD20), macrophages (CD68 and Ber-Mac 3) and natural killer cells (CD56) in the rectal mucosa of patients with bacillary dysentery and in healthy controls were studied in an effo...
Dr. Md. Shahidul Islam, Saul Tzipori, Md. Rayhanul Islam, Alf A. Lindberg
A rapid and sensitive method for the detection of Shigella dysenteriae type 1 and Shigella flexneri serotypes in faeces based on capture of the bacteria with immunomagnetic particles is described. The particles were coated with either of two different monoclonal antibodies specific for the O-antigen...
Dilara Islam, Bengt Wretlind, Lennart Hammarström, Birger Christensson et al.
Systemic and mucosal antibody responses during shigellosis were analysed and correlated with clinical severity. Patients infected with Shigella dysenteriae type 1 (SDIP), and S. flexneri (SFIP) were grouped according to disease severity. The disease was more severe in SDIP than in SFIP. Higher level...
Rubhana Raqib, Bengt Wretlind, Alf A. Lindberg, Å. Ljungdahl et al.
Sameera Sayeed, Sack Da, Firdausi Qadri
The plasmid profile of P. shigelloides SVC 01, strain with cross-reactivity with Sh. sonnei, (obtained from Dr Alf Lindberg, Stockholm University, Sweden), was assayed and compared with other local isolates of Plesiomonas spp. and also with Sh. sonnei. Diversity in occurrence of plasmids among diffe...