Alaullah Sheikh, Richelle C. Charles, Sean M. Rollins, Jason B. Harris et al.
BACKGROUND: Salmonella enterica serotype Paratyphi A is a human-restricted cause of paratyphoid fever, accounting for up to a fifth of all cases of enteric fever in Asia. METHODOLOGY/PRINCIPAL FINDINGS: In this work, we applied an RNA analysis method, Selective Capture of Transcribed Sequences (SCOT...
Richelle C. Charles, Alaullah Sheikh, Bryan Krastins, Jason B. Harris et al.
Salmonella enterica serotype Typhi is the cause of typhoid fever and a human-restricted pathogen. Currently available typhoid vaccines provide 50 to 90% protection for 2 to 5 years, and available practical diagnostic assays to identify individuals with typhoid fever lack sensitivity and/or specifici...
Peng Xu, Mohammad Murshid Alam, Anuj Kalsy, Richelle C. Charles et al.
Bacterial O-SP-core antigens can be conjugated to proteins in the same, simple way as synthetic, linker-equipped carbohydrates by applying squaric acid chemistry. Introduction of spacers (linkers) to either O-SP-core antigens or protein carriers, which is involved in commonly applied protocols, is n...
Chandrabali Ghose, Anuj Kalsy, Alaullah Sheikh, Julianne E. Rollenhagen et al.
Clostridium difficile is the leading cause of nosocomial infectious diarrhea. C. difficile produces two toxins (A and B), and systemic and mucosal anti-toxin A antibodies prevent or limit C. difficile-associated diarrhea. To evaluate whether transcutaneous immunization with formalin-treated C. diffi...
Alaullah Sheikh, Richelle C. Charles, Nusrat Sharmeen, Sean M. Rollins et al.
Background: Salmonella enterica serotype Typhi is the cause of typhoid fever. It is a human-restricted pathogen, and few data exist on S. Typhi gene expression in humans.
Julianne E. Rollenhagen, Anuj Kalsy, Francisca Cerda, Manohar John et al.
Toxin-coregulated pilin A (TcpA) is the main structural subunit of a type IV bundle-forming pilus of Vibrio cholerae, the cause of cholera. Toxin-coregulated pilus is involved in formation of microcolonies of V. cholerae at the intestinal surface, and strains of V. cholerae deficient in TcpA are att...
Mohammad Murshid Alam, Megan Kelly Bufano, Peng Xu, Anuj Kalsy et al.
BACKGROUND: Protective immunity against cholera is serogroup specific. Serogroup specificity in Vibrio cholerae is determined by the O-specific polysaccharide (OSP) of lipopolysaccharide (LPS). Generally, polysaccharides are poorly immunogenic, especially in young children. METHODOLOGY: Here we repo...
Alaullah Sheikh, Farhana Khanam, Md. Abu Sayeed, Taibur Rahman et al.
BACKGROUND: Salmonella enterica serotype Typhi is a human-restricted intracellular pathogen and the cause of typhoid fever. Cellular immune responses are required to control and clear Salmonella infection. Despite this, there are limited data on cellular immune responses in humans infected with wild...
Julianne E. Rollenhagen, Anuj Kalsy, Rina Saksena, Alaullah Sheikh et al.
Antibodies specific for Vibrio cholerae lipopolysaccaride (LPS) are common in humans recovering from cholera, and constitute a primary component of the vibriocidal response, a serum complement-mediated bacteriocidal response correlated with protection against cholera. In order to determine whether t...
Abdullah A. Tarique, Anuj Kalsy, Mohammad Arifuzzaman, Sean M. Rollins et al.
A shortcoming of currently available oral cholera vaccines is their induction of relatively short-term protection against cholera compared to that afforded by wild-type disease. We were interested in whether transcutaneous or subcutaneous boosting using a neoglycoconjugate vaccine made from a synthe...
Mohammad Murshid Alam, Lillian L. Tsai, Sean M. Rollins, Alaullah Sheikh et al.
Salmonella enterica serotype Paratyphi A is a human-restricted pathogen and the cause of paratyphoid A fever. Using a high-throughput immunoscreening technique, in vivo-induced antigen technology (IVIAT), we identified 20 immunogenic bacterial proteins expressed in humans who were bacteremic with S....
Md Saruar Bhuiyan, Anuj Kalsy, Mohammad Arifuzzaman, Richelle C. Charles et al.
Transcutaneous vaccination can induce both mucosal and systemic immune responses. However, there are few data on anti-polysaccharide responses following transcutaneous vaccination of polysaccharides, despite the role that anti-polysaccharide responses play in protecting against intestinal mucosal an...
Anuj Kalsy, Omar Faruk Bhuiyan, Richelle C. Charles, Md Arifuzzaman et al.
Abstract Vaccination induces both mucosal & systemic immune response. Polysaccharide antigen (PAg) which is T cell independent antigen does not stimulate production of memory responses. Cholera holotoxin (CT) is shown to be a potent immunoadjuvant. Transcutaneous immunization (TCI) is recently s...