Comparative evaluation of in vivo relative bioavailability and in vitro bioaccessibility of arsenic in leafy vegetables and its implication in human exposure assessment

Arsenic (As) contamination in vegetables is a severe threat to human health. However, the evaluation of As relative bioavailability (As-RBA) or bioaccessibility in vegetables is still unexplored. The study sought to evaluate the As-RBA in commonly consumed ten leaf vegetables collected from As-polluted farmlands. Additionally, the As-RBA was determined using rat bioassay and compared with As bioaccessibility through five commonly used in vitro methods, including UBM (Unified BARGE Method), SBRC (Solubility Bioavailability Research Consortium), DIN (Deutsches Institut für Normung e.V.), IVG (In Vitro Gastrointestinal), and PBET (Physiologically Based Extraction Test). Results showed that the As-RBA values were 14.3-54.0% among different vegetables. Notably, significant in vivo-in vitro correlations (IVIVC) were observed between the As-RBA and the As bioaccessibility determined by the…