Sheng Yang, Yanping Cheng, Zaozao Chen, Tong Liu et al.
Nanoplastics, including polystyrene nanoplastics (PS-NPs), are widely existed in the atmosphere, which can be directly and continuously inhaled into the human body, posing a serious threat to the respiratory system. Therefore, it is urgent to estimate the potential pulmonary toxicity of airborne NPs...
Zaozao Chen, Ning Ma, Xiaowei Sun, Qiwei Li et al.
Three-dimensional in vitro tumor models provide more physiologically relevant responses to drugs than 2D models, but the lack of proper evaluation indices and the laborious quantitation of tumor behavior in 3D have limited the use of 3D tumor models in large-scale preclinical drug screening. Here we...
Qiwei Li, Zaozao Chen, Ying Zhang, Shuang Ding et al.
Current techniques for visualizing and quantifying cellular forces have limitations in live cell imaging, throughput, and multi-scale analysis, which impede progress in cell force research and its practical applications. We developed a photonic crystal cellular force microscopy (PCCFM) to image vert...
Sheng Yang, Zaozao Chen, Yanping Cheng, Tong Liu et al.
Environmental pollution is a widespread problem, which has seriously threatened human health and led to an increase of human diseases. Therefore, it is critical to evaluate environmental pollutants quickly and efficiently. Because of obvious inter-species differences between animals and humans, and ...
Yanping Cheng, Zaozao Chen, Sheng Yang, Tong Liu et al.
The extensive production and use of nanomaterials have resulted in the continuous release of nano-sized particles into the environment, and the health risks caused by exposure to these nanomaterials in the occupational population and the general population cannot be ignored. Studies have found that ...
Sheng Yang, Tianyi Zhang, Yiling Ge, Yanping Cheng et al.
Nanoplastics are prevalent in the air and can be easily inhaled, posing a threat to respiratory health. However, there have been few studies investigating the impact of nanoplastics on lung injury, especially chronic obstructive pulmonary disease (COPD). Furthermore, cell and animal models cannot de...
Uwe Marx, Sonja Beken, Zaozao Chen, Eva‐Maria Dehne et al.
The regular t4 workshops on biology-inspired microphysiological systems (MPS) have become a reliable benchmark for assessing fundamental scientific, industrial, and regulatory trends in the MPS field. The 2023 workshop participants concluded that MPS technology as used in academia has matured signif...
Kai Huang, Mingyue Li, Qiwei Li, Zaozao Chen et al.
Patient-derived organoids have proven to be a highly relevant model for evaluating of disease mechanisms and drug efficacies, as they closely recapitulate in vivo physiology. Colorectal cancer organoids, specifically, exhibit a diverse range of morphologies, which have been analyzed with image-based...
Shiyi Tan, Yan Ding, Wei Wang, Jianhua Rao et al.
AI image processing techniques hold promise for clinical applications by enabling analysis of complex status information from cells. Importantly, real-time brightfield imaging has advantages of informativeness, non-destructive nature, and low cost over fluorescence imaging. Currently, human liver or...
Yiling Ge, Sheng Yang, Tianyi Zhang, Kai Luo et al.
The novel pollutant, N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine quinone (6-PPDQ) leaked out of the tire and has attracted extensive concerns due to its high lethal toxicity of salmon. However, the potential hepatotoxicity of 6-PPDQ exposure and its mechanisms are unknown. As a novel 3D cell ...
Yiling Ge, Sheng Yang, Tianyi Zhang, Jie Li et al.
• Provide the first experimental comparison of PS-NPs-induced liver injury via oral and inhalation exposure. • PS-NPs caused wide lipid profiles alterations, with oral and inhalation exposure showed specialties. • Lipidomic and transcriptional analysis were integrated to explore exposure route-depen...
Wentao Zhao, Zilin Zhang, Shihui Xu, Xuan Sun et al.
culture of cancer cells and drug screening are key technical components in functional oncology precision medicine. However, most studies primarily focus on constructing models using established cell lines, with limited integration with clinical diagnosis or patient treatment. This review provides a ...
Wei Hou, Zilin Zhang, Jing Zhang, Shihui Xu et al.
In recent decades, in addition to monolayer-cultured cells, three-dimensional tumor spheroids have been developed as a potentially powerful tool for the evaluation of anticancer drugs. However, the conventional culture methods lack the ability to manipulate the tumor spheroids in a homogeneous manne...
Yu Yao, Xuan Du, Y J Li, Yuchen Ma et al.
Introduction: Abnormal pigmentation plays an important role in various skin diseases and in studies of whitening efficacy.Three-dimensional pigmented epidermis-on-a-chip models provide a crucial in vitro platform for exploring melanin production and regulation in skin. However, dynamic and non-invas...
Qihang Yang, Yuening He, Shuo Wang, Z.T. Li et al.
Microvascular network formation is governed by a variety of factors, with interstitial flow (IF) playing a pivotal role. However, the impact of multidirectional IF (MDIF) on microvascular network development remains insufficiently explored. In this study, we developed a platform consisting of a Squa...